IS_MIRRI21 aims to implement the Microbial Resource Research Infrastructure (MIRRI) and secure its long-term sustainability. With this in mind, the mission of IS_MIRRI21 is to serve Bioscience and Bioindustry by providing (a) a broad range of high-quality biological resources and associated data, (b) long-term sustainability of microbial biodiversity, and (c) knowledge and professional development. This mission will be achieved by offering users from academia, governmental laboratories and the private sector access to a portfolio of microbial Biological Resource Centres ́ (mBRC’s), services, expertise, education and training build-up and synergistically on the top of mBRC’s proficiencies. To accelerate innovative research processes in life sciences and biotechnology using microorganisms, or their derivatives, IS_MIRRI21 will set up a single-entry point portal (Collaborative Work Environmental) to promote its services, resources, expertise and knowledge transfer activities, as well as to provide access to partners ́ resource associated data made interoperable for data searching and data mining. The continuously increasing knowledge will be transferred to users via expert clusters (e.g., for legal framework, for biosecurity, to support training and education programmes, information and cut edge technology, etc.). It will promote cross-disciplinary and innovative solutions (industry and academic) research collaboration through the transnational access offer (pilot study) in different microbial topics defined in pipeline services. With this project IS_MIRRI21 envisages to be a sound partner of the ESFRI landscape on the Biological and Medical Sciences Research Infrastructures, consolidate the operation of MIRRI and enlarge the membership and European coverage and beyond.