Coordonnées
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Email : gwenaelle.andre-leroux@inrae.fr DR2, PhD, HDR Adresse : INRA - Unité MaIAGE |
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I work as a structural computational biologist at Inra, on the Campus of Jouy-en-Josas, in MaIAGE: Applied Mathematics and Computer Science, from Genome to Environment. This lab is headed by Dr. S. Schbath, and has a co-sharing divisions of MIA (Applied Mathematics and Informatics) and MICA (Microbiology and Food Chain). I belong to the StatInfoMics team headed by Dr. P. Nicolas.
My interests deal with computational screening of whole genomes and metagenomes to infer proteins structures and functional annotations. I have special focus on proteins of the gut microbiome which are involved in biomedical properties such as host-pathogen or anti-inflammatory signaling. My primary interest deals with prokaryotic signaling through phosphorylation and dephosphorylation events, mediated by protein Ser/Thr kinases and protein Tyr phosphatases, respectively. I also have fruitful collaborations with biologists from Micalis-INRA, IPBS Toulouse or collaborators from South America and I aim to infer on the combo sequence/structure/function/dynamics for the analysis of their favorite protein(s).
For all my projects, i combine structural and computational bioinformatics, molecular modeling expertise, X-ray crystallography skills, with in vitro/in vivo data from my collaborators, whose methods span biophysics, biochemistry, cellular biology, neurobiology, and immunology.
<a href="https://www.researchgate.net/profile/Gwen_Andre">Gwen André on ResearchGate</a>
https://www.linkedin.com/in/gwena%C3%ABlle-andr%C3%A9-leroux-61aa43184/
Main collaborations
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Faculdad de Ciencias, Montevideo, Uruguay. Coll. A. Villarino & M. Berois. Inra MaIAGE M. Mariadassou. Projet Ecos-Sud Uruguay 2015: Characterization of the Tyrosine -phosphatase of the intracellular pathogen ORF-virus.
- Unidad de Cristalografía de Proteínas. Instituto de Biología Molecular y Celular de Rosario IBR-CONICET-UNR, Argentina. M-N. Lisa. In silico characterization of the conservation of a signaling pathway mediated by PknG in Actinobacteria.
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Institut Pasteur, Structural Microbiology Unit, Dir. Prof. P. Alzari. Coll. P. Alzari. Structural and functional characterization of PknB, essential Ser/Thr protein kinase of Mycobacterium tuberculosis. http://www.pasteur.fr/en/research/structural-biology-chemistry/units-gr….
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Institut de Pharmacologie et de Biologie Structurale, Coll. H. Marrakchi & M. Daffé. Toulouse. Structural and functional characterization of enzymes responsible for the biogenesis and degradation of mycolic acids in M. tuberculosis.
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Institut Pasteur, Human genetics and Cognitive Functions Unit, Dir. Prof. T. Bourgeron. Coll. I. Cloez-Tayarani. Towards structural insights on CNTN6 and Shank3.
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INRA Micalis PIMS. Dr. N. Rama Rao, COMBAC Dr. V. Monnet, ProbiHôte Dr. JM. Chatel & P. Langella.
Coordination projects
@2019-2021 Coordinator of ECOS-Sud project: Structural and functional characterization of Mfd/UvrA interaction.
@2019-2021 Coordinator in partnership with N. Rama Rao of a TWB project
@2015- 2018 Coordinator of MEM INRA MetaFoldScan
@2015-2017 Coordinator of ECOS-Sud project: Structural and functional characterization of DUSP Orf virus.
Background
2012 - Senior Scientist -structuralist- at INRA Jouy-en-Josas, MaIAGE SIO.
2004-2012 Senior scientist -structuralist- detached at Institut Pasteur Paris, Structural Microbiology Unit. Skills in X-ray crystallography.
2000-2004 Junior scientist INRA Nantes, BIA. Structural bioinformatics.
1999-2000 Cornell University, Ithaca, USA. Post-doc in Molecular dynamics J.W. Brady's lab at https://foodscience.cals.cornell.edu/people/j-brady
1994-1998 INRA, Nantes. PhD in Molecular Mechanics and modeling.
Publications 2016-2020*
* Verplaetse E., André-Leroux, G., Duhutrel, P., Coeuret, G., Chaillou, S;, Nielsen-Leroux, C., Champommier-Vergès, M.C. Heme Uptake in Lactobacillus sakei Evidenced by a New Energy Coupling Factor (ECF)-Like Transport System. Appl. Environ. Microbiol. 2020 86 (18)
* Huillet, E., Bridoux, L., Barboza, I., Lemy, C., André-Leroux, G., & Lereclus, D. The Signaling Peptide PapR is required for the Activity of the Quorum-Sensor PlcrA in Bacillus thuringiensis. Microbiology 2020 166 (4): 398-410.
* Tran, S.L., Cormontagne, D., Vidic, J., André-Leroux, G., & Rama Rao, N. Structural Modeling of Cell Wall Peptidase CwpFM (EntFM) Reveals Distinct Intrinsically Disordered Extensions Specific to Pathogenic Bacillus cereus Strains. Toxins (Basel) 2020: 12 (9).
* Millerand, M., Sudre, L., Nefla, M., Pène, F., Rousseau, C., Pons, A;, Ravat, A., André-Leroux, G., Akira, S., Satoh, T., Berenbaum, F., Jacques, C. Osteoarthritis Cartilage 2020. 28 (5): 646-657.
* Henry, C., Haller L., Blein-Nicolas, M. Zivy, M., CANETTE, A., Verbrugghe, M., Mezange, C., Boulay, M., Gardan, R., Samson S. A., Martin, V., André-Leroux, G & Monet, V. Identification of Hanks-type kinase PknB-specific Targets in the Streptococcus thermophilus Phosphoproteome. Frontiers Microbiol. ,2019 10, 1319:1-17
* Wagner, T*., André-Leroux, G*.,Hindie, V., Barilone, N., Lisa, M-N., Le Normand, B., O’Hare, H., Bellinzoni M & Alzari, P. Structural insights into the functional versatility of a FHA domain protein in mycobacterial signaling. Science Signaling 2019, 12 (580). Doi: 10.1126/scisignal.aav9504. * equal contribution
* Lisa, M-N., Cvirkaite-Krupovic, V., Richet, E., André-Leroux, G., Alzari, P., HAOUZ, A., Danot O. Belt and Suspenders: the Double Autoinhibition Mechanism of Signal Transduction ATPases with Numerous Domains (STAND) with a Tetratricopeptide Repeat Sensor. NAR 2019 doi: 10.1093/nar/gkz112 .
* Gouder, L., Vitrac, V., Goubran-Botros, H., Danckaert, A., Tinevez, J-Y., André-Leroux, G., Atanasova, E., Lemière, N., Biton, A., Leblond, C., Poulet, A., Boland, A., Deleuze, J-F., Benchoua, A., Delorme, R., Bourgeron, T. and Cloez-Tayarani, I. Altered Spinogenesis in iPSC-derived Cortical Neurons from Patients with Autism carrying de novo SHANK3 Mutations. Scientific Reports 2019 9, 94.
* Segovia, D., Haouz, A., Porley, D., Olivero, N., Martinez, M., Mariadassou, M., Berois, M., André-Leroux, G., Villarino, A. OH1 from Orf virus: a new tyrosine phosphatase that displays distinct structural features and triple substrate specificity. Journal of Molecular Biology 2017, 26, 429.
* A. Collucia, G. La Regina, N. Barilone, M.N. Lisa, A. Brancale, G. André-Leroux, P.M. Alzari, Silvestri, R. Structure-based virtual screening to get news scaffold inhibitors of the Ser/Thr Protein Kinase PknB from Mycobacterium tuberculosis. Letters in Drug Design and Discovery, 2016, 13 (10) : 1012-1018.
* N. H. Le, V. Molle, N. Eynard, M. Miras, A. Stella, F. Bardou, S. Galladrin, V. Guittet, G. André-Leroux, M. Bellinzoni, P. Alzari, L. Mourey, O. Burlet-Schiltz, M. Daffé, H. Marrakchi. Ser/Thr Phosphorylation regulates the Fatty Acyl-AMP ligase Activity of Fad32, an Essential Enzyme in Mycolic Acid Biosynthesis. J. Biol. Chem. 2016, 291 (43): 22793-22805.
* O. Mercati, G. Huguet, A. Danckaert, G. André-Leroux, A. Maruani, M. Bellinzoni, T. Rolland, L. Gouder, A. Mathieu, J. Buratti, F. Amsellem, M. Benabou, J. Van-Gils, A. Beggiato, M. Konyukh, J.P. Bourgeois, M.J. Gazzellone, R.K. Yuen, S. Walker, M. Delépine, A. Boland, B. Régnault, M. Francois, T. Van Den Abbeele, A. L. Mosca-Boidron, L. Faivre, Y. Shimoda, K. Watanabe, D. Bonneau, M. Rastam, M. Leboyer, S. W. Scherer, C. Gillberg, R. Delorme, I. Cloëz-Tayarani, T Bourgeron. CNTN6 mutations are Risk Factors for Abnormal Auditory Sensory Perception in Autim Spectrum Disorders. Mol Psychiatry., 2016. doi: 10.1038/mp.2016.61.
* M. N. Schroeder*, M. V. Tribulatti*,J. Carabelli, G. André-Leroux, J. J. Caramelo, V. Cattaneo & O. Campetella. Characterization of a double-CRD-mutated Gal-8 recombinant Protein that retains Costimulatory activity on antigen-specific T cell response. Biochemical Journal 2016, 473 (7) 887-898.