Titre du projet
Nature et rôle des moteurs biotiques dans la construction des communautés microbiennes des fromages traditionnels
Name of the call for tenders
France Génomique
F. Irlinger (INRA, Grignon) ; C. Delbès (INRA, Aurillac)
Participants de MaIAGE
A.-L. Abraham, V. Loux, M. Mariadassou, O. Rué
INRA GMPA, INRA URF, INRA MICALIS, Genoscope, RMT Fromages de Terroir, CNAOL , CNIEL
Beginning and end of the year
Year of end
Multi "omic" approaches will be combined to investigate the factors shaping the microbial communities in milks and traditional cheeses. Firstly, microbial diversity at species level will be explored across the 45 French PDO milks and cheeses, by metabarcoding sequencing. Secondly, focusing on six keystone species, the genomes of contemporary strains and of strains isolated from cheese up to several decades ago or from other biotopes will be compared in search of genetic traits of microbial species adaptation to the cheese context. Finally, profiling of microbial communities will be carried out by shotgun sequencing at the key stages of the manufacturing processes to understand the settling of keystone species and their specific gene expression in the time scale of cheese-making.
Année de soumission